Formula
- Use the units shown beside each field.
- Results update automatically whenever you change an input.
- Displayed values use up to four decimal places, or scientific notation for very small or large numbers. Calculations use standard floating-point arithmetic; exact integer and fraction tools identify their own precision rules.
- Pure DNA mass-ratio arithmetic; reaction conditions, biological sequence design and ligation success are outside scope.
Example Calculation
- Vector mass: 50 ng
- Vector length: 3000
- Insert length: 1000
- Insert/vector molar ratio: 3
Vector mass = 50; Vector length = 3000; Insert length = 1000; Insert/vector molar ratio = 3. Primary result: 50.
Frequently asked questions
How do I use this calculator?
Enter vector mass, vector length, insert length, insert/vector molar ratio. The result updates automatically. Use Reset to restore the example values.
What method does it use?
insert mass=vector mass ×insert/vector lengths ×molar ratio.. Pure DNA mass-ratio arithmetic; reaction conditions, biological sequence design and ligation success are outside scope.
Why might a rounded result differ?
The calculation keeps full numeric precision internally, then rounds the displayed result. Rounding intermediate steps by hand can produce a slightly different answer.
Are my inputs saved online?
No. This calculator processes your inputs in your browser. A recently used list stores only calculator names on this device, not your entered values.
Check the assumptions and units before using this result. Read about calculation methods.