Formula
- Use the units shown beside each field.
- Results update automatically whenever you change an input.
- Displayed values use up to four decimal places, or scientific notation for very small or large numbers. Calculations use standard floating-point arithmetic; exact integer and fraction tools identify their own precision rules.
- Assumes a linear initial-rate interval and blank-corrected product formation. One U converts one micromole per minute under the stated assay conditions.
Example Calculation
- Product formed: 12 µmol
- Reaction time: 3 min
- Enzyme sample volume: 0.2 mL
12 µmol in 3 minutes is 4 U; a 0.2 mL sample has 20 U/mL.
Frequently asked questions
How do I use this calculator?
Enter product formed, reaction time, enzyme sample volume. The result updates automatically. Use Reset to restore the example values.
What method does it use?
U=product micromoles/time minutes; activity concentration=U/sample mL.. Assumes a linear initial-rate interval and blank-corrected product formation. One U converts one micromole per minute under the stated assay conditions.
Why might a rounded result differ?
The calculation keeps full numeric precision internally, then rounds the displayed result. Rounding intermediate steps by hand can produce a slightly different answer.
Are my inputs saved online?
No. This calculator processes your inputs in your browser. A recently used list stores only calculator names on this device, not your entered values.
Check the assumptions and units before using this result. Read about calculation methods.