Formula
- Use the units shown beside each field.
- Results update automatically whenever you change an input.
- Displayed values use up to four decimal places, or scientific notation for very small or large numbers. Calculations use standard floating-point arithmetic; exact integer and fraction tools identify their own precision rules.
- Uses the conventional 50 ng/µL per A260 at 1 cm for dsDNA. Not for RNA/ssDNA; contamination and instrument corrections affect accuracy.
Example Calculation
- Blank-corrected A260: 0.2
- Dilution factor: 10
- Optical path length: 1 cm
Blank-corrected A260 = 0.2; Dilution factor = 10; Optical path length = 1. Primary result: 100.
Frequently asked questions
How do I use this calculator?
Enter blank-corrected a260, dilution factor, optical path length. The result updates automatically. Use Reset to restore the example values.
What method does it use?
dsDNA ng/µL=A260 ×50 ×dilution/path cm.. Uses the conventional 50 ng/µL per A260 at 1 cm for dsDNA. Not for RNA/ssDNA; contamination and instrument corrections affect accuracy.
Why might a rounded result differ?
The calculation keeps full numeric precision internally, then rounds the displayed result. Rounding intermediate steps by hand can produce a slightly different answer.
Are my inputs saved online?
No. This calculator processes your inputs in your browser. A recently used list stores only calculator names on this device, not your entered values.
Check the assumptions and units before using this result. Read about calculation methods.